CSIR & Reliance Industries to develop RT-LAMP based test for Covid-19

RT-LAMP a rapid, accurate and cost-effective test

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CSIR
RT-LAMP a rapid, accurate and cost-effective test can be done with indigenous components and set up with minimal expertise and instrumentation

As part of the Covid-19 mitigation mission of Country, CSIR has strategized its R&D to develop, integrate, scale-up, and deploy necessary technological interventions for combating Coronavirus pandemic in the country. Considering the multifarious problems created by the coronavirus, which require interventions, the CSIR under the guidance of its director-general, Shekhar Mande has formed five verticals to coordinate various research activities into digital and molecular surveillance, drugs & vaccines, rapid and economical diagnostics, hospital assistive devices & PPEs, and supply chain and logistics.

Since testing is a vital component in Covid-19 mitigation, CSIR-IIIM, Jammu a constituent laboratory of CSIR has partnered with Reliance Industries (RIL) to develop and scale-up a new Reverse Transcriptase-Loop Mediated Isothermal Amplification (RT-LAMP) based Covid-19 diagnostic kit for which a formal MOU has also been signed between CSIR-IIIM, Jammu and RIL.

Covid-19 RT-LAMP test is a nucleic acid-based test carried out from nasal or throat swab samples from patients. The test recipe has been developed and successfully demonstrated using synthetic templates. It is rapid (45-60 min), cost-effective and accurate test.

It has been tested with a small number of patient samples, and validating the kit on more patient samples is planned and will be done together with RIL.

The advantage of this test is that the RT-LAMP based Covid-19 kit components are easily available, and these can be manufactured entirely in India. While real-time PCR does the current Covid-19 testing their components are mostly imported. Further, these tests are expensive; require highly trained workforce, costly instruments and a relatively high-end lab and cannot be deployed at remote locations in quarantine centers, airports and railway stations.

On the other hand, the RT-LAMP test can be done in a single tube with minimal expertise in a basic lab setup like mobile units or kiosks for testing at airports, railway stations, bus stands and other public places. The end detection of the test is a simple colored reaction, which is easily visible in UV light, and now is being modified such that it can be detected in regular light.

After testing the accuracy of the kit, on a much larger number of patients, CSIR-IIIM and RIL will jointly approach ICMR for approval. RIL plans to rapidly scale up the tests for the broader population and use it for easy, rapid, and widespread diagnosis for Covid-19 detection for society’s more substantial interest.

With the formal launch of the RT-LAMP based diagnostic test, the Covid-19 testing will be more rapid, cheap, easy and accessible and would go a long way quickly isolating the infected individuals and mitigating the spread of virus.

Ram Vishwakarma, director and Sumit Gandhi, principal scientist from CSIR-IIIM and Santanu Dasgupta, senior vice president, R&D and Manish Shukla, general manager R&D from RIL side are monitoring this project.

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